PCR-TIES (Third Irrelevant Enzyme Site)-Mediated Gene Fusion Method
نویسندگان
چکیده
منابع مشابه
Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems.
We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding approach using conventional recombinant DNA methodologies and two-component ligations failed to give...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96201bm13